Chromosomal dynamism in progeny of outbreak-related sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM

Article Abstract:

Pulsed-field gel electrophoresis (PFGE) was successfully used to identify hemorrhagic colitis and hemolytic-uremic syndrome (HUS) outbreaks caused by sorbitol-fermenting (SF) enterohemorrhagic colitis Escherichia coli (EHEC) O157:NM. The findings indicate that since the human body contains other prophage-inducing agents, such as H2O2 released by neutrophilis, strains with a single stx2 gene might produce large amounts of stx2 in vivo, thereby promoting their ability to cause HUS.

All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Sorbitol, Causes of, Risk factors, Escherichia coli infections, Hemolytic-uremic syndrome

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Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli

Article Abstract:

The Vero cell cytotoxicity assays, enzyme immunoassays (EIA), latex agglutination (LA), and colony immunoblot assays to characterize Shiga toxin (Stx) production in non-O157 Escherichia coli clinical isolates possess an stx(sub 2) variant as their sole stx gene. It is concluded that six of 37 non-O157 Esherichia coli strains possessing stx 2 gene variant stx(sub 2d) or stx(sub 2e) secreted no detectable Stx.

Genetic transcription, Transcription (Genetics), Microbial toxins

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Subtypes of the plasmid-encoded serine protease EspP in Shiga toxin-producing Escherichia coli: Distribution, secretion, and proteolytic activity

Article Abstract:

The prevalence, distribution and structure of EspP (extracellular serine protease, plasmid encoded) in Shiga toxin-producing Escherichia coli (STEC) are investigated. It is concluded that espP is widely distributed among STEC strains and displays genetic heterogeneity.

Proteolysis

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