Cis-acting determinants affecting centromere function, sister-chromatid cohesion and reciprocal recombination during meiosis in Saccharomyces cerevisiae
Article Abstract:
A study of the role of conserved centromere DNA elements CDEI, CDEII and CDEIII in meiotic chromosome disjunction fidelity by testing the effect of 13 different centromere mutations on meiotic centromere activity using a system with homologous pairs of human DNA derived yeast artificial chromosomes (YACs) as marker chromosomes reveals high fidelity segregation of YACs with a wild-type CEN DNA sequence in meiosis I and meiosis II. Enhanced levels of meiosis I nondisjunction and precocious sister-chromatid segregation occur in YACs with a 31-bp deletion mutation in centromere DNA element II in a monosomic, homocentric or heterocentric YAC pair configuration.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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Modulation of Saccharomyces cerevisiae DNA double-strand break repair by SRS2 and RAD51
Article Abstract:
A study of the mechanism of RAD52-mediated DNA double-strand break repair in Saccharomyces cerevisiae by screening for partially active RAD52 allele-suppressing genes reveals that a phenotypic null allele of SRS2 suppresses multiple RAD52 and RAD51 multiple alleles but not the rad52-Delta or rad51-delta mutations. RAD52 allele-suppression by srs2 differs from RAD51 overexpression in its mechanism, since both effects are additive. The positive influence of RAD52 and RAD51 on recombinational repair mechanisms is indicated by the elevated recombination-dependent sensitivity of an srs2-Delta RAD51 strain.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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Mismatch correction acts as a barrier to homeologous recombination in Saccharomyces cerevisiae
Article Abstract:
A study of the influence of mismatch repair genes PMS1, MSH2 and MSH3 on recombination fidelity in Saccharomyces cerevisiae using a homeologous mitotic recombination assay reveals that inactivation of MSH2 and MSH3 results in partial reestablishment of the recombination rates associated with homologous sequences. The rate of homologous recombination in the msh2 and msh3 double mutant is 30% of the homologous rate in a wild-type background. Homologous recombination is not enhanced in pms1 deletion mutants.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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