Multiple elements and auto-repression regulate Rox1, a repressor of hypoxic genes in Saccharomyces cerevisiae
Article Abstract:
A study of the regulatory elements in yeast Saccharomyces cerevisiae reveals the presence of four such elements. RNA blot analysis and the use of a ROX1/lacZ fusion construct indicate that the Rox1 gene that codes for a heme-activated repressor of hypoxic genes suppresses its own expression. ROX1 is also regulated by the heme-dependent transcriptional activator Hap1, while anaerobic expression is suppressed by an Ssn6/Tup1-dependent repressor. The Hap1-independent aerobic expression of ROX1 in the Delta-hap1 deletion strains and the anaerobic Rox1 expression in Delta-Tup1 strains involve an unknown transcriptional activator.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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Regulation of gene expression by oxygen in Saccharomyces cerevisiae
Article Abstract:
The oxygen-regulation of expression in two classes of genes in Saccharomyces cerevisiae is described. The genes in which regulation is heme-dependent are classified as either heme-activated or heme-repressed. Heme activation is triggered by the HAP1 protein or by the HAP2/3/4 complex. Heme repression, on the other hand, is characterized by the involvement of the ROX1 repressor. Several anaerobic genes with heme-independent expression which were repressed by oxygen were also discussed. Heme-independent repressions were also found to have two different regulatory mechanisms.
Publication Name: Microbiological Reviews
Subject: Biological sciences
ISSN: 0146-0749
Year: 1992
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Extra telomeres, but not internal tracts of telomeric DNA, reduce transcriptional repression at Saccharomyces telomeres
Article Abstract:
Genetic experiments reveal that introduction of extra telomeres on a linear plasmid or expression of higher levels of a deletion derivative of Rap1-delta-BBp reduces the fraction of cells showing telomere position effect (TPE) in Saccharomyces. Reduction of TPE due to expression of Rap1-delta-BBp is suppressed by the overexpression of Sir3p, while this overexpression has no influence on the reduction of TPE induced by the addition of extra telomeres. Experimental data support the presence of a terminus-specific binding protein in Saccharomyces, which is vital for TPE.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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- Abstracts: A simple and reliable method for hybridization of homothallic wine strains of Saccharomyces cerevisiae. Genetic instability of heterozygous, hybrid, natural wine yeasts
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