Cloning of a creatinase gene from Pseudomonas putida in Escherichia coli by using an indicator plate
Article Abstract:
An alternative strategy to screen large numbers of transformants for creatinase activity was developed. The procedure involves the coculture of transformants with Proteus mirabilis to utilize its urea-hydrolyzing ability as an inducer of color change in indicator plates. The procedure was used to screen an Pseudomonas putida NTU-8 genomic library, resulting in the isolation of a 2.7-kb DNA fragment coding for creatinase activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Engineering of solvent-tolerant Pseudomonas putida S12 for bioproduction of phenol from glucose
Article Abstract:
An entirely biobased production process for the conversion of a renewable substrate (glucose) into the toxic bulk chemical phenol via the central metabolite tyrosine is achieved in the solvent-tolerant strain Pseudomonas putida S12. The method results in accumulation of 58 mM phenol in the octanol phase and there is a twofold increase in the overall production compared to a single-phase fed batch.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Chromosomal locus for cadmium resistance in Pseudomonas putida consisting of a cadmium-transporting ATPase and a MerR family response regulator
Article Abstract:
Pseudomonas putida contains two proteins that confer resistance to cadmium. CadA is similar to cadmium-transporting ATPases in gram-positive bacteria and CadR is similar to the MerR family involved in mercury detoxification. CadA is induced by cadmium, lead, and zinc whereas CadR is only induced by cadmium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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- Abstracts: Recombination-dependent mutation in Escherichia coli occurs in stationary phase. Genetic recombination in Escherichia coli: disputation at Cold Spring Harbor, 1946-1996
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