Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides

Article Abstract:

Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes functionally expressed in Escherichia coli, reveal a 50-amino acid conserved region, possibly involved in disaccharide binding. The translated sequence for the genes shows a highly conserved region within the EIIC domain which may be the site for binding and phosphorylation. Phosphotransferase system genes on plasmids of Klebsiella oxytoca encode enzymes with a broad range of substrates and support the most rapid growth of recombinant Escherichia coli on cellobiose-minimal medium.

Analysis, Evaluation, Carbohydrates, Bacterial genetics, Gene expression

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Use of a genetically engineered Escherichia coli strain to produce 1,2-dihydroxy-4'-chlorobiphenyl

Article Abstract:

A genetically engineered Escherichia coli strain was used to produce 1,2-dihydroxy-4'-chlorobiphenyl (DHCB) from 4-chlorobiphenyl (4-CBP). This mutant strain contains a plasmid coding for biphenyl dioxygenase and dihydrodiol dehydrogenase and lacks the ability to produce 3-phenylcathechol dioxygenase. Conditions affecting the conversion of DHCB from 4-CBP were identified and analyzed. Results show that biologic synthesis of DHCB using mutated E. coli strains can be an alternative to the expensive chemical methods commonly used to synthesize this compound.

Production management, Usage, Microbial genetic engineering, Microbiological synthesis, Organochlorine compounds, Organic chlorine compounds

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