Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-L-gluconate, a precursor of L-ascorbic acid, in a recombinant G.oxydans strain
Article Abstract:
The recombinant strain constructed by the cloning of the genes for L-sorbose dehydrogenase (SDH) and L-sorbosone dehydrogenase (SNDH) from Gluconobacter oxydans T-100, produces 2-keto-L-gulonate (2-KLGA) from D-sorbitol. The high level production of 2-KLGA, a precursor of L-ascorbic acid is done by simple fermentation of the recombinant Gluconobacter. DNA sequencing show that the SDH and SNDH are coded in sequential open reading frames with 1,497 and 1,599 nucleotides respectively. The DNA is used to obtain an expression vector, pSDH155, which produces 2-KLGA from D-sorbitol in G. oxydans G624.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Biodegradation of cyclohexylamine by Brevibacterium oxydans IH-35A
Article Abstract:
A bacterial strain that can grow on cyclohexylamine has been synthesized using enrichment and isolation methods. The strain, IH-35A, belongs to the genus Brevibacterium oxydans and induces the cyclohexylamine oxidase, cyclohexanone monooxygenase and 6-hexanolactone hydrolase activities by growth on cyclohexylamine. Cyclohexylamine degradation is induced by cyclohexanone, 6-hexanolactone, 6-hydroxyhexanoate and adipate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Biotransformation of patulin by gluconobacter oxydans
Article Abstract:
A study conducted showed that a bacterium isolated from patulin-contaminated apples was capable of degrading patulin to a less-toxic compound ascladiol. The observations showed degradation up to 96% of patulin in apple juices containing up to 800 [mu]g/ml of patulin and incubated with Gluconobacter oxydans.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Construction of engineered bifunctional enzymes and their overproduction in Aspergillus niger for improved enzymatic tools to degrade agricultural by-products
- Abstracts: Searching chromatin for stem cell identity. Intergenic transcription through a polycomb group response element counteracts silencing
- Abstracts: Influence of sporulation medium composition on transcription of ger operons and the germination response of spores of Bacillus cereus ATCC 14579
- Abstracts: Cataloging life. Minding your $ and cents: how to manage personal finances and plan for retirement on a scientist's budget
- Abstracts: A mechanism of resistance to hydrogen peroxide in Vibrio rumoiensis S-1. Bacterial genes responsible for the biosynthesis of eicosapentaenoic and docosahexaenoic acids and their heterologous expression