Construction of engineered bifunctional enzymes and their overproduction in Aspergillus niger for improved enzymatic tools to degrade agricultural by-products
Article Abstract:
Two chimeric enzymes, FLX and FLXLC, are constructed and successfully overproduced in Aspergillus niger. The results have shown that the fusion of naturally free cell wall hydrolases and an A. niger-derived carbohydrate-binding module (CBM) onto bifunctional enzymes enables the increase of the synergistic effect on the degradation of complex substrates.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Design and production in Aspergillus niger of a chimeric protein associating a fungal feruloyl esterase and a clostridial dockerin domain
Article Abstract:
A chimeric enzyme associating feruloyl esterase A from Aspergillus Niger and dockerin from Clostridium thermocellum is produced. The conclusion states that translational fusion to glucoamylase improved the secretion efficiency of a himeric Doc-FAEA protein and allowed production of the first functional fungal enzyme joined to a bacterial dockerin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger
Article Abstract:
The study describes the gene overexpression and the biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger. The substance is found as a by-product in apple mare and coffee pulp.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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