Cloning, sequencing, and expression of genes encoding phosphotransacetylase and Acetate kinase from Clostridium acetobutylicum ATCC 824
Article Abstract:
The genes encoding phosphotransacetylase (PTA) and acetate kinase (AK) from Clostridium acetobutylicum are cloned and sequenced to find the features of these enzymes. The AK and PTA show elevated activities on cell extracts from Escherichia coli and C. acetobutylicum harboring the subclone. The pta and ack genes lie near the pta upstream in the clostridial chromosome. While pta gene encodes a protein of 333 amino acid residues, ack encodes a polypeptide of 401 residues. The pta gene has an operon arrangement with a single transcriptional start site lying 70 bp upstream of the start codon.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824
Article Abstract:
Non-replicative integrational plasmids containing butyrate kinase (buk) and phosphotransacetylase (pta) gene fragments were used to inactivate buk and pta in Clostridium acetobutylicum ATCC 824. Inactivation of the pta gene on the chromosome reduced pta and acetate kinase activity as well as acetate production, while inactivation of the buk gene minimized buk activity and butyrate production but increased butanol production.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Expression of abrB310 and sinR, and effects of decreased abrB310 expression on the transition from acidogenesis to solventogenesis, in clostridium acetobutylicum ATCC 824
Article Abstract:
A single homologue to sinR and three highly similar homologues to abrB, designated abrB310, abrB1941, and abrB3647, in Clostridium acetobutylicum ATCC 824 are identified. A demonstration that the promoters of abrB1941 and abrB3646 were not active under the growth conditions tested using reporter vectors is presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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