A Clostridium difficile gene encoding flagellin

Article Abstract:

Research demonstrates that flagellin gene sequences from two virulent strains of Clostridium difficile exhibit 91% inter-strain identity. Data further show that the gene product is a 31 kilodalton protein and the flagellin has a molecular mass of 39 kilodalton.

United Kingdom, Analysis, Genetic aspects, Nucleotide sequence, Base sequence, Genomes, Flagella (Microbiology), Flagella

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GroEL (Hsp60) of Clostridium difficile is involved in cell adherence

Article Abstract:

GroEL (Hsp60) of Clostridium difficile has been found to be involved in cell adherence in a study on whether the GroEL heat shock protein is implicated in the adherence of C. difficile to tissue culture cells, augmented by stresses. The 1623 bp groEL gene is highly conserved between some C. difficile isolates. The C. difficile groELS operon was isolated from two strains. Characterization and expression of the hsp60 gene was also carried out. PCR amplification of the groEL gene waS used with RFLP analysis.

Physiological aspects, Bacteria, Pathogenic, Pathogenic bacteria, Heat shock proteins

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Clostridium difficile cell attachment is modified by environmental factors

Article Abstract:

Research was conducted to examine the effect of environmental stresses on Clostridium difficile adherence in vitro in the Vero cell adherence model by the use of either various cultures or adherence assay conditions. The reference strain used in all of the adherence assays was the toxigenic C difficile isolate 79-685. Results indicate that Vero cells have the ability to bind to one, two or three bacterial surface proteins depending on the bacterial culture conditions.

Environmental engineering

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