Colonial opacity variations among the choleragenic vibrios

Article Abstract:

Stereoscopy and transmitted oblique illumination were used to characterize the cultures of Vibrio cholerae 01, biotype El Tor and V. cholerae serogroup O139 collected from ongoing outbreaks in the Western Hemisphere and in India and Bangladesh, respectively. Examination of the original cultures revealed colonial heterogeneity with about 10 distinguishable degrees of opacity while freshly isolated strains from patients and those which were rapidly and carefully stored were found to be more homogeneous although with some opacity differences as well. Such reversible changes in colonial opacity were found to be associated with changes in bacterial virulence and phenotypic expressions.

Usage, Causes of, Electrophoresis, Electron microscopy, Bacteriology, Vibrio infections, Bacterial cultures, Stereoscope, Stereoscopes

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Characterization and possible functions of a new filamentous bacteriophage from Vibrio cholerae O139

Article Abstract:

A filamentous phage '493' from a Vibrio cholerae O139 strain AJ27-493 was purified and partially characterized. The phage was inactive on classical biotype V. cholerae O1 but it was active on El Tor biotype strains isolated before 1994 when El-Tor reemerged in Bangladesh. The phage was also active on O139 strains. The receptor for 493 was not TcpA, unlike the filamentous ctx(sub phi). The phage genome was a 9.3 kb closed circular single-stranded molecule with a 0.4 kb double-stranded stem supporting a 2 kb single-stranded loop.

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A novel filamentous phage, fs-2, of Vibrio cholerae O139

Article Abstract:

Research was conducted to isolate fs-2, a novel filamentous bacteriophage, from Vibrio cholerae O139 strain MDO14. The plaque formation activity achieved through heating was controlled through chloroform treatment. During this activity, the bacteriophage's DNA genome transformed into a double-stranded replicative form in the host cell. The genetic organization of other filamentous phages were found to be preserved.

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