Comparison of commercially available kits with standard methods for the detection of coliforms and Escherichia coli in foods
Article Abstract:
The standard method of the US Food and Drug Administration and the Colilert fluorogenic substrate-based detection method show a high detection sensitivity for coliforms and Escherichia coli present in food. The detection rate of the microorganisms by a test using eosine methylene blue is half that of a test using violet red bile 4-methylumbelliferyl-beta-D-glucuronide (MUG) agar. The Colilert method is more sensitive than the other commercially available MUG-based methods. The detection sensitivity of the beta-glucuronidase enzyme reaction is good.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Comparison of commercially available kits with standard methods for detection of Salmonella strains in foods
Article Abstract:
The detection of Salmonella serovars in foods by six commercial kits is comparable to the U.S. Food and Drug Administration (USFDA) methods and the Japanese standard method. However, only the USFDA method and immunomagnetic separation coupled with the xylose-lysine-brilliant green agar method (MS-XLBG) positively detect Salmonella serovars in artificially inoculated foods. The USFDA method detects all seven wild-type Salmonella species whereas the MS-XLBG detects salmonellae from six samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Comparison of a fluorogenic assay with a conventional method for rapid detection of Vibrio parahaemolyticus in seafoods
Article Abstract:
A comparison of the conventional method and the fluorogenic assay of seafood samples reveals the presence of Vibrio parahaemolyticus in some and trypsin-like activity in others. Vibrio alginolyticus exhibits intracellular trypsin like activity, which is observed when the initial density of V. parahaemolyticus reaches a value more than 100 per gram. Fluorogenic assay differentiates between V. parahaemolyticus and other bacteria with the isolation of sucrose negative colonies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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- Abstracts: Oxidative stress detection with Escherichia coli harboring a katG'::lux fusion. Responses to toxicants of an Escherichia coli strain carrying a uspA'::lux genetic fusion and an E. coli strain carrying a grpE'::lux fusion are similar
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