Oxidative stress detection with Escherichia coli harboring a katG'::lux fusion
Article Abstract:
An Escherichia coli strain containing a plasmid composed of the E. coli katG promoter, fused to the Vibrio fischeri lux operon, produces luminescence when exposed to hydrogen peroxide (H2O2), organic peroxides, cigarette smoke and ethanol. The expression of the plasmid is controlled by the oxyR regulon. The luminescence produced in the presence of ethanol and H2O2 is much greater than that produced in the presence of only ethanol or H2O2. The plasmid can be used as a biosensor, to determine the oxidant and antioxidant properties of chemicals, and to study the cellular response of cells to oxidative stress.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids
Article Abstract:
The detection of DNA damage by use of Escerichi coli carrying reporter plasmids was examined to present the construction and initial characterization of an alternative panel of easily assayed transcriptional fusions. The study described promoter fusions that allow the detection of sublethal levels of DNA-damaging agents. Results show that the agents tested can cause damage to DNA by other assays making induction of lux fusions by these treatments a common occurrence.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Responses to toxicants of an Escherichia coli strain carrying a uspA'::lux genetic fusion and an E. coli strain carrying a grpE'::lux fusion are similar
Article Abstract:
Toxicity induction in Escherichia coli in response to chemical toxicants, when regulated by a uspA'::lux fused gene, exhibits clear distinctions among toxicants. This differential response is useful to detect toxicants. Toxicant response of this gene is similar to that of the thermal shock-responsive gene fusion grpE'::lux, except that the grpE'::lux strain exhibits a greater magnitude of induction.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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