Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water
Article Abstract:
PCR amplification and hybridization give maximum sensitivity for the specific detection of Cryptosporidium parvum and Giardia lamblia in water samples. The optimization of primer annealing temperature and MgCl2 concentrations yields the specificities of primer pairs, which are either species or genus specific. The simultaneous detection of the two pathogenic protozoa by multiplex PCR demonstrates the amplification of 256- and 163-bp products from the 18S rRNA gene of C. parvum and heat shock protein gene of G. lamblia, respectively.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Crytosporidium parvum
Article Abstract:
An in vitro infectivity assay for Cryptosporidium parvum was developed by utilizing cell culture combined with reverse transcriptase-polymerase chain reaction (RT-PCR) to detect infectious organisms. The method also utilizes PCR primers that are specific for Cryptosporidium parvum to detect oocysts in environmental water samples. Furthermore, the need for multiple or nested reactions was eliminated by the spin column purification of extracted DNA from the environmental water samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Randomly amplified polymorphic DNA PCR analysis of bovine Cryptosporidium parvum strains isolated from the watershed of the Red River of the North
Article Abstract:
Cryptosoporidium parvum causes cryptosporidiosis and although this protozoal disease is hypothesized to be zoonotic, it has never been proven. Sixteen bovine C. parvum isolates from the Red River of the North and two bovine and two human isolates from Australia were analyzed using randomly amplified polymorphic DNA polymerase chain reaction to determine relationships among these strains analysis of Cryptosporidium. Results reveal that these isolates belong to four different strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Effect of pasteurization on infectivity of Cryptosporidium parvum oocysts in water and milk. In vitro interactions of Asian freshwater clam (Corbicula fluminea) hemocytes and Cryptosporidium parvum oocysts
- Abstracts: Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR
- Abstracts: The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313
- Abstracts: Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity. Motor neuron-derived retinoid signaling specifies the subtype identity if spinal motor neurons
- Abstracts: Genetic organization of the mle locus and identification of a mleR-like gene from Leuconostoc oenos. Molecular characterization of the gene encoding an 18-kilodalton small heat shock protein associated with the membrane of Leuconostoc oenos