Construction of a Helicobacter pylori-Escherichia coli shuttle vector for gene transfer in Helicobacter pylori
Article Abstract:
A research was conducted to study the cryptic plasmid pHP489 used as a base replicon to construct plasmid vectors for Helicobacter pylori, to assess whether the shuttle vector could transfer manipulated gene in Escherichia coli into H. pylori. Electroporation was utilized to transform plasmid DNAs into H. pylori while H. pylori was grown on a brucella agar plate. Results showed that E. coli plasmids did not replicate and were not maintained in H. pylori.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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A simple filtration technique to detect enterohemorrhagic Escherichia coli O157:H7 and its toxins in beef by multiplex PCR
Article Abstract:
A simple two-step filtration approach is proposed for enterohemorrhagic Escherichia coli O157:H7 detection in beef through multiplex PCR. The method addressed the inhibition of the PCR by food particles and unknown metabolic byproducts of bacteria. The approach was found to be more effective than other assays in detecting and characterizing Escherichia coli O157:H7 directly from food.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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