A 12*7 kb fragment of the Mycobacterium tuberculosis genome is not present in Mycobacterium bovis
Article Abstract:
Experimental evidence shows that both Mycobacterium bovis and Mycobacterium bovis BCG have no 12*7 kb region found in the genome of Mycobacterium tuberculosis. This was gleaned from Southern blotting, polymerase chain reaction and sequence analysis experiments conducted on Mycobacterium bovis. An analysis of the 12*7 kb fragment indicates that it represents a deletion in Mycobacterium bovis rather than an insertion in Mycobacterium tuberculosis. This fragment might prove useful as a target for a polymerase chain reaction test to distinguish Mycobacterium bovis from Mycobacterium tuberculosis.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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Differential transcription of the MPB70 genes in two major groups of Mycobacterium bovis BCG substrains
Article Abstract:
A study of the sequence of MPB70 genes from BCG Tokyo and Pasteur by Northern hybridization analysis and reverse transcriptase-PCR shows that differential abilities of the transcription, mediate the secretion of MPB70 antigen protein. The upstream and the downstream region of both the MPB70 strains are identical and the amount of MPB70 protein depends upon the presence of MPB64 gene. The experimental set up of cloning of MPB70 genes of Tokyo and Pasteur is discussed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Antisense RNA to ahpC, an oxidative stress defence gene involved in isoniazid resistance, indicates that AhpC of Mycobacterium bovis has virulence properties
Article Abstract:
Mycobacterial oxidative stress response is of interest in research associated with resistance to isoniazid (INH), a drug used in human tuberculosis treatment. Certain INH-resistant M. tuberculosis strains have mutations in the ahpC promoter, leading to increased expression of AhpC. An antisense RNA approach has been developed to reduce levels of AhpC, confirming the utility of antisense RNA technology in mycobacterial virulence investigations.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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