Quantitative molecular assay for fingerprinting microbial communities of wastewater and estrogen-degrading consortia
Article Abstract:
A quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-RFLP) assay, is developed for simultaneous determination of microbial diversity and abundance within a complex community. The results indicate that the real-time-t-RFLP assay can be a powerful molecular tool for learning about microbial communities in various engineered systems and natural habitats.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Molecular characterization of Brevibacillus lacterosporus and its potential use in biological control
Article Abstract:
Different PCR-based methods that were used to examine the genetic variability of thirty-three strains of Brevibacillus laterosporus including three novel strains isolated from Brazilian soil samples are discussed. Characterization of Brevibacillus laterosporus by different molecular methods reveals an elevated level of genetic polymorphism within the species, indicating that the isolates are clonally related.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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