Contraselectable streptomycin susceptibility determinant for genetic manipulation and analysis of Helicobacter pylori
Article Abstract:
A two-gene cassette that can be selected both, against, because of a Campylobacter jejuni rpsL gene and for, due to an erm gene (erythromycin resistance) is presented. The desired Erm (super s) STR (super r) were acquired at a range of frequencies from 17 to 96% among STr (super r) transformants, with the Erm (super s) yield apparently depending on the strain background and genome location of the targeted locus.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Atypical helicobacter canadensis strains associated with swines
Article Abstract:
In The Netherlands and Denmark forty-two Helicobacter isolates were isolated from swine feces and all twelve isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis (99% similarity). Amplified fragment length polymorphism genomic typing showed that isolates recovered from swine feces were genetically distinct from H. canadensis strains obtained from humans and geese.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Direct quantification of Campylobacter jejuni and Campylobacter lanienae in feces of cattle by real-time quantitative PCR
Article Abstract:
Campylobacter jejuni and Campylobacter lanienae in feces of cattle can be directly quantified using nested real-time quantitative (RTQ)-PCR method. The effectiveness of the RTQ-PCR method in quantifying Campylobacter jejuni and Campylobacter lanienae is proved using SYBR green.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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- Abstracts: Spectrum of the reductive dehalogenation activity of Desulfitobacterium frappieri PCP-1. Purification, cloning, and sequencing of a 3,5-dichlorophenol reductive dehalogenase from Desulfitobacterium frappieri PCP-1
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- Abstracts: New threshold and confidence estimates for terminal restriction fragment length polymorphism analysis of complex bacterial communities. part 2