Conversion of xylan to ethanol by ethanologenic strains of Escherichia coli and Klebsiella oxytoca
Article Abstract:
Genetic engineering of ethanologenic strains of Escherichia coli and Klebsiella oxytoca has enabled these cells to ferment xylan to ethanol via a two-stage process. The xylanase gene from Clostridium thermocellum was fused with the N terminus of lacZ and incorporated into the genome of both strains. This ethanol production of both organisms was associated with intracellular accumulation of xylanase. Release of this xylanase to a xylan solution creates a saccharified hydrosylate that can again be fermented to ethanol by the same organism. This fermentation replenishes xylanase supply, thereby, creating a cycle.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum
Article Abstract:
The PET genes for ethanol production of Zymomonas mobilis was integrated into Klebsiella oxytoca M5A1. The best construct, K. oxytoca P2, could efficiently convert cellobiose to ethanol. It was able to metabolize cellobiose and cellotriose, eliminating the need for beta-glucosidase. The addition of plasmids to this integrated construct also allowed the production of thermostable endoglucanase D as a coproduct, reducing the requirement for commercial cellulases during the cellulose fermentation to ethanol.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli
Article Abstract:
A genetically engineered strain of Escherichia coli efficiently ferments acid hydrolysates of pine cellulose and hemicellulose into ethanol. The strain was genetically mutated to eliminate succinate production and contains Zymomonas mobilis genes for ethanol production. Use of this E. coli strain yields more ethanol than conventional yeast fermentation processes. This high yield is due to more efficient sugar conversion utilizing all component hexoses and pentoses.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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