"Cre"-ating mouse mutants - a meeting review on conditional mouse genetics

Article Abstract:

No one fully developed kit of genetic reagents for making conditional alterations in genes has been developed, but site-specific recombinase Cre can take out DNA sequences between two loxP recognition sequences very efficiently in mice or in a culture of mammal cells. To make a mouse mutation takes more than making a simple knockout. Complex genome engineering strategies are needed to knock out or misexpress a gene as wanted, rearrange the chromosomal context of a gene, or make allilic alterations. Development of the right tissue-specific Cre-expressing lines that have great specificity combined with high-level expression will be the key in the future. Centralized repositories and distribution for strains of mice that can be used widely are needed.

Germany, France, Methods, Genetic engineering, Gene expression, Genetic recombination, Genetically modified mice

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Diethylstilbestrol regulates trophoblast stem cell differentiation as a ligand of orphan nuclear receptor ERR(beta)

Article Abstract:

Diethylstilbestrol (DES), the synthetic estrogen, has been found to regulate trophoblast stem cell differentiation. DES has been shown to promote coactivator release from orphan nuclear receptor ERR(beta) and inhibits transcriptional activity. Treating trophoblast stem cells with DES pushed them toward differentiation toward the polyploid giant cell lineage. A novel pathway for DES action has been defined. Evidence for steroidlike control of trophoblast development was found.

Statistical Data Included, Cytochemistry, Cell differentiation, Stem cells, Trophoblast, Trophoblasts, Diethylstilbestrol

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Combinatorial effects of Flk1 and Tal1 on vascular and hematopoietic development in the mouse

Article Abstract:

Research has been conducted on Flk1 and Tal1 which fail to form endothelial Flk1 and Tal1 nd hematopoietic cells in mouse embryo mutant. The authors test the hypothesis that Flk1 and Tal1 regulate cell fate choice in early development into endothelial, smooth muscle and hematopoutic lineages.

Science & research, Analysis, Usage, Gene mutations, Gene mutation, Cells (Biology), Developmental biology, Cells, Genetic research, Combinatorial analysis, Hematopoietic agents

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