DNA polymerase epsilon links the DNA replication machinery to the S phase checkpoint

Article Abstract:

Damage-uninducible mutant allele, dun2, of DNA polymerase epsilon (pol-epsilon) is defective in S phase checkpoint and thus relates the DNA replication machinery to S phase checkpoint. Mutation analysis and interallelic complementation reveal the presence of two individual but important domains, a C-terminal polymerase domain and an N-terminal polymerase domain in pol-epsilon. The DNA pol-epsilon directs the cell cycle and transcriptional responses to replication block and functions as a sensor of DNA replication.

Cell cycle, DNA polymerases

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Visualization of chromosomal domains with boundary element-associated factor BEAF-32

Article Abstract:

In Drosophila, the cDNA of the boundary element-associated factor (BEAF)-32 encodes for a protein which binds to scs' but not to scs and is involved in controlling the boundary function of scs'. BEAF has a localized association with many chromosomes and is thus involved in regulating the functioning of the many boundary elements which define the boundary of the independently functioning genome domains. BEAF-32 differs structurally from the other known proteins and structural motifs.

Drosophila, Chromosomes, Chromosomal proteins

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Visualization of replication factories attached to a nucleoskeleton

Article Abstract:

A homogenous population of replication sites was studied by concentrating HeLa cells in early S phase, encapsulating these with agarose microbeads, permeabilizing them with streptolysin O and incubating the cells in a physiologic buffer with biotin-11-d UTP. DNA synthesis sites were then immunolabeled. Electron microscopy revealed that labeled DNA moves through a replication factory while being attached to a nucleoskeleton.

DNA

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