Degradation of morpholine by an environmental Mycobacterium strain involves a cytochrome P-450
Article Abstract:
Research was performed as regards the isolation and characterization of a Mycobacterium strain that metabolizes morpholine as the sole source of carbon, nitrogen and energy. 1H nuclear magnetic resonance was used to quantitatively monitor the degradation of morpholine, to identify the intermediates and to assist in proving the role played by cytochrome P-450 in the degradation. Results revealed that the inhibition of morpholine degradation relied on the morpholine/metyrapone ratio and that P-450 catalyzes the cleavage of the C-N bond of the morpholine ring.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Morpholine degradation pathway of Mycobacterium aurum MO1: direct evidence of intermediates by in situ 1H nuclear magnetic resonance
Article Abstract:
A study was conducted to assess the degradation of morpholine by Mycobacterium aurum MO1 through the use of in situ 1H nuclear magnetic resonance spectroscopy. Such a method allows the identification and quantification of metabolites which accumulated during growth by rendering invisible the fully deuterated microbial cultures. It also facilitated the study of the degradative metabolism of M. aurum MO1 by directly analyzing the incubation medium in water.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Genetic and molecular oganization of the alkylbenzene catabolism operon in the psychotrophic strain Pseudomonas putida 01G3
Article Abstract:
A potential regulator and six putative enzymes were determined for the alkylbenzene upper pathway within the psychotrophic organism Pseudomonas putida 01G3. The regulator, EbdR, and enzymes, EbdAaAbAcAdBC, demonstrated pronounced similarities with P. putida RE204 enzymes in the equivalent pathway.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Detection and characterization of broad-host-range plasmids in environmental bacteria by PCR. Resuscitation by ferrioxamine E of stressed Salmonella enterica serovar Typhimurium from soil and water microcosms
- Abstracts: Genetic manipulation of polyphosphate metabolism affects cadmium tolerance in Escherichia coli. Coordinated, differential expression of two genes through directed mRNA cleavage and stabilization by secondary structures
- Abstracts: The Vibrio cholerae O1 chromosomal integron. Genetic relationships between clinical and environmental Vibrio cholerae isolates based on multilocus enzyme electrophoresis
- Abstracts: A new broad-spectrum protease inhibitor from the entomopathogenic bacterium Photorhabdus luminescens. Occurrence of natural dixenic associations between the symbiont Photorhabdus luminescens and bacteria related to Ochrobactrum spp. in tropical entomopathogenic Heterorhabditis spp. (Nematoda, Rhabditida)
- Abstracts: Characterization of thermophilic consortia from two souring oil reservoirs. Direct cloning from enrichment cultures, a reliable strategy fo isolation of complete operons and genes from microbial consortia