Degradation of nitrobenzene by a Pseudomonas pseudoalcaligenes
Article Abstract:
A metabolic pathway causes the breakdown of nitrobenzene to nitrosobenzene, and later to hydroxylaminobenzene. Each step requires grown cells, under aerobic and anaerobic conditions, and transforms nitorbenzene to 2-aminophenol with the oxidation of 2 mol of NADPH. Ring cleavage, requiring ferrous iron, gave a transient yellow product with a maximum A-380. In the presence of NAD, the product vanished and NADH was obtained. The absence of NAD led to the spontaneous conversion of the ring fission product to picolinic acid.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction
Article Abstract:
Toluene-grown cells of Pseudomonas putida F1 and Pseudomonas sp. JS150 oxidized 2- and 3-nitrotoluene to 2- and 3-nitrobenzyl alcohol. These results indicate that toluene dioxygenase was responsible for oxidizing the methyl substituent. Incubation of these cells with 4-nitrotoluene resulted in production of 2-methyl-5-nitrophenol and 3-methyl-6-nitrocatechol. A possible mechanism for this reaction was elucidated, with oxidation by toluene dioxygenase as the initial step.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Initial reductive reactions in aerobic microbial metabolism of 2,4,6-trinitrotoluene
Article Abstract:
Research was performed on Pseudomonas sp. clone and trinitrotoluene (TNT)-8 and TNT-32 to determine the significance of the 2,4,6-trinitrotoluene pathway. Evidence suggests that initial microbial transformations of 2,4,6-TNT are characterized largely by reductive rather than oxidation reactions due to high electron deficiency. Furthermore, formation of hydride complexes could not be identified with the TNT-enriched strains of TNT-8 and TNT-32 or with Pseudomonas clone A.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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- Abstracts: Transformation of 2,4,6-trinitrotoluene by Pseudomonas pseudoalcaligenes JS52. Oxidative transformation of aminodinitrotoluene isomers by multicomponent dioxygenases
- Abstracts: Limited degradation of chlorophenols by anaerobic sludge granules. Reductive dehalogenation of chlorophenol by Desulfomonile tiedjei DCB-1
- Abstracts: Degradation of 2,4-dinitrotoluene by the lignin-degrading fungus Phanerochaete chryssosprorium. Degradation of azo dyes by the lignin-degrading fungus Phanerochaete chrysosporium
- Abstracts: Utilization of 3-chloro-2-methylbenzoic acid by Pseudomonas cepacia MB2 through the meta fission pathway. Degradation of 2-methylbenzoic acid by Pseudomonas cepacia MB2
- Abstracts: Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction