Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification
Article Abstract:
Amplification of 16S rRNA from mycoplasmal organisms using the polymerase chain reaction allowed the identification of species- and genus-specific sequences suitable for identifying the organisms. The species-specific sequences hybridized only with the corresponding species. The genus-specific sequence was highly specific for mycoplasma but also cross-reacted with some related organisms. The V2, V3, V5 and V7 regions of the 16S rRNA were used to develop the species-specific primers, while the genus-specific primer was obtained from nucleotides 1029 to 1055.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Application of a Mycoplasma group-specific PCR for monitoring decontamination of Mycoplasma-infected Chlamydia sp. strains
Article Abstract:
Incubation with Triton X-100 is useful in the decontamination of the laboratory strains of Chlamydia pneumoniae, C. pecorum and C. trachomatis, infected with Mycoplasma. A Mycoplasma group-specific PCR technique detects the contamination of cell lines. The contamination with Mycoplasma spp. changes the experiment parameters of Chlamydia strains and necessitates a Mycoplasma-free material. Antibodies cannot be used against mycoplasma as they suppress the Chlamydia cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction
Article Abstract:
The detection of Campylobacter spp. in chicken products usinga combination of short enrichment culturee and polymerase chain reaction (PCR) is discussed. This involved the development of a specific PCR and probe hybridization assay based on the 16S rRNA gene sequence. A comparison of the method with conventional detection techniques yielded the same results. However, the results from the PCR-culture assay was obtained faster.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Nested allele-specific PCR primers distinguish genetic groups of Uncinula necator. PCR assays that identify the grapevine dieback fungus Eutypa lata
- Abstracts: Displacement of sequence-specific transcription factors from mitotic chromatin
- Abstracts: In situ identification of cyanobacteria with horseradish peroxidase-labeled, rRNA-targeted oligonucleotide probes
- Abstracts: Fate of residual lignin during delignification of kraft pulp by Trametes versicolor. Lignin peroxidase activity is not important in biological bleaching and delignification of unbleached kraft pulp by Trametes versicolor
- Abstracts: Chromosome conservation in the Bovidae. Identification of an autosome to X chromosome translocation in the domestic cow