Detection of aflatoxigenic molds in grains by PCR
Article Abstract:
The use of selected genes, present in the aflatoxin biosynthesis pathway, in the PCR is effective in the detection of aflatoxigenic molds inhabiting stored grains. The ver-1, omt-1 and apa-2 are the genes involved in the aflatoxin biosynthetic pathway. The amplification occurs only in the DNA of the Aspergillus parasiticus and Aspergillus flavus in all the primer pairs of the three genes. The inoculation of sterile corn with other molds fails to amplify the DNA even at the highest inoculum level.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Purification of a 40-kilodalton methyltransferase active in the aflatoxin biosynthetic pathway
Article Abstract:
The conversion of sterigmatocystin (ST) to O-methylsterigmatocystin (OMST) is the last level in the aflatoxin bosynthetic pathway of the filamentous fungi Aspergillus flavus and A. parasiticus. Five soft-gel chromatographic steps were used to carry out the purification of the reaction catalyst, S-adenosylmethionine-dependent methyltransferase. A second enzyme that can convert ST to OMST in vitro was characterized from the purification process.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Detection of noroviruses in tap water in Japan by means of a new method for concentrating enteric viruses in large volumes of freshwater
- Abstracts: Chromosome conservation in the Bovidae. Identification of an autosome to X chromosome translocation in the domestic cow
- Abstracts: The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway
- Abstracts: Adhesion of germlings of Botrytis cinerea. Composition and enzymatic activity of the extracellular matrix secreted by germlings of Botrytis cinerea
- Abstracts: The recombination hotspot khi is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme