The recombination hotspot khi is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme
Article Abstract:
The RecBCD enzyme is proven to degrade dsDNA asymmetrically during the process of DNA unwinding. Moreover, this nucleolytic activity is also attentuated after interacting with recombination hotspot khi, while a pause of the RecBCD enzyme at this site implies a representation of both the recognition event and the time involved in altering the enzymatic functional state. Thus, the khi sequence serves as a regulatory element or allosteric effector that alters the RecBCD enzyme's recombinational characteristics.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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A molecular throttle: the recombination hotspot chi controls DNA translocation by the RecBCD helicase
Article Abstract:
Research points out that RecBCD helicase/nuclease is involved in the initiation of homologous recombination at double-stranded DNA breaks and that its interaction with chi affects translocation by RecBCD. Data indicate that RecBCD pauses precisely at chi during its translocation along DNA molecules. Furthermore, interaction with chi uncouples the RecD motor from RecBCD enzyme resulting in its slower translocase activity.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2003
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The recombination hot spot chi is embedded within islands of preferred DNA pairing sequences in the E. coli genome
Article Abstract:
Hot spots are loci that display elevated frequencies of exchange of homologous DNA. Chi (5'-GCTGGTGG-3') is a hot spot in E coli whose importance is underscored by the large number present in its genome. Results of a study indicate that Chi is surrounded by almost a kilobase of TGG-enriched DNA. Moreover, the findings suggest that GT-rich regions may be used universally to stimulate homologous recombination.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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