Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction
Article Abstract:
Polymerase chain reaction (PCR) technology applied to the study of soils and sediments enabled the detection oflow numbers of bacterial cells. However, the sensitivity of PCR detection on DNA extracted from environmental soils and sediments was low due to the presence of humic substances or other components which inhibited the polymeraseactivities or binding of primers. Dilution of these limiting substances loweredtheir concentration but it also reduced PCR detection limit.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction
Article Abstract:
A rapid gel filtration method to separate DNA from extracts containing humic substances followed by polymerase chain reaction (PCR) was used. The purification of crude DNA extracts with Sephadex G-200 columns greatly reduced the inhibitory effects of humic acid and increased the sensitivity of detection by the PCR. This technique could thus be applied to sediments containing a low content of humic substances.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
PCR-restriction fragment length polymorphism method for detection of Cyclospora cayetanensis in environmental waters without microscopic confirmation
Article Abstract:
The authors have investigated Cyclospora cayetanensis in environmental water samples. They describe the development of the alternative netsted-PCR-restriction fragment length polymorphism protocol for detection of the bacterium which differentiates C. cayetanensis amplified target sequence from those that may cross-react.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry. Comparison of cellular and biomass specific activities of dominant bacterioplankton groups in stratified waters of the Celtic Sea
- Abstracts: Detection of the light organ symbiont, Vibrio fisheri, in Hawaiian seawater by using lux gene probes. Characterization of htrB and msbB mutants of the light organ symbiont Vibrio fischeri
- Abstracts: Identification of two proline transport systems in Staphylococcus aureus andtheir possible roles in osmoregulation
- Abstracts: Detection of viable Giardia cysts by amplification of heat shock-induced mRNA. Persistence of viruses in desert soils amended with anaerobically digested sewage sludge
- Abstracts: Helix 4 mutants of the Bacillus thuringiensis insecticidal toxin Cry1Aa display altered pore-forming abilities