Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry
Article Abstract:
An assay was designed to measure protein biomass of marine planktonic bacteria by flow cytometry. The procedure was calibrated by employing five species of Bacteria isolated from seawater samples and grown in culture at various temperatures. The intensity of SYPRO-protein fluorescence of these bacteria strongly correlated with their total protein content, measured by the bicinchoninic acid method to be in the range of 60 to 330 fg of protein/cell.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Flow sorting of marine bacterioplankton after fluorescence in situ hybridization
Article Abstract:
The objective of the study was to develop a protocol for flow sorting hybridized prokaryotes, and to explore the usefulness of this protocol for aquatic microbial ecology. Results demonstrate that a combination of catalyzed fluorescent reporter deposition (CARD-FISH) and flow sorting can be useful for studying the diversity and the activity and the genomes of different bacterial populations in aquatic habitats.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Comparison of cellular and biomass specific activities of dominant bacterioplankton groups in stratified waters of the Celtic Sea
Article Abstract:
A clade of alpha-proteobacteria related to Roseobacter spp., a Cytophaga-Flavobacterium cluster, and a cluster of gamma-proteobacteria produce most of the total bacterioplankton metabolic activity in the Celtic Sea. The smallest cells of the gamma-proteobacteria had the highest growth-specific rates, accounting for their dominance in nutrient-limited waters.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr. Enhanced production of insulin-like growth factor I fusion protein in Escherichia coli by coexpression of the down-regulated genes identified by transcriptome profiling
- Abstracts: Effects of light on the microcystin content of Microcystis strain PCC 7806. Determination of oligopeptide diversity within a natural population of Microcystis spp. (Cyanobacteria) by typing single colonies by matrix-assisted laser desorption ionization-time of flight mass spectrometry
- Abstracts: Transcription-dependent recombination and the role of fork collision in yeast rDNA. The hairless gene mutated in congenital hair loss disorders encodes a novel nuclear receptor corepressor
- Abstracts: Divergence of melanocortin pathways in the control of food intake and energy expenditure. Defects in adaptive energy metabolism with CNS-linked hyperactivity in PGC-1 alpha null mice