Determination of left-right asymmetric expression of nodal (ital) by a left side-specific enhancer with sequence similarity to a lefty-2 (ital) enhancer
Article Abstract:
Similar transcription mechanisms seem to be behind asymmetric expression of nodal (ital), a gene expressed on the left side of developing mouse embryos and implicated in left/right (L/R) axis formation, and also of lefty-2 (ital) and of the expression earlier of nodal (ital) in endoderm and epiblasts. The transcriptional regulatory element of nodal responsible for the asymmetric expression of the gene has been identified. Intron 1 contains a left side-specific enhancer (ASE) that can direct transgene expression in the lateral plate mesoderm and prospective floor plate on the left.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1999
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lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal
Article Abstract:
Developing mice deficient in the gene lefty-1 are monitored to investigate the function of this gene in the development of laterality. Results reveal that mice lacking this gene developed left-to-right positional anomalies in the visceral organs. The most prominent of these positional defects was thoracic left isomerism. Further, the absence of lefty-1 resulted in the bilateral expression of nodal, lefty-2 and Pitx2, the expression of which is normally limited to the left. It is concluded that lefty-1 restricts the expression of these genes on the left side of the embryo.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
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Distinct transcriptional regulatory mechanisms underlie left-right asymmetric expression of lefty-1 and lefty-2
Article Abstract:
Studies show that different regulatory mechanisms are responsible for lefty-1 and lefty-2 gene expression in mice embryos. Despite their similarities, the two genes are expressed through a left side-specific enhancer for lefty-2 and a combination of a right side-specific silencer and bilateral enhancers for lefty-1. These processes account for their asymmetric expression.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
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