Development and field performance of a broad-spectrum nonviable asporogenic recombinant strain of Bacillus thuringiensis with greater potency and UV resistance
Article Abstract:
The development of, and field trials conducted on, a novel recombinant Bacillus thuringiensis strain that produces large amounts of two different crystal proteins are reported. It was demonstrated that encapsulation of the toxins within the cell protects them from deactivation by UV radiation. The strain was found to be a sporulation-deficient mutant that cannot survive in the environment, thus, minimizing any environmental effects that arise from the dissemination of large numbers of viable spores.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Genetic analysis of cryIIIA gene expression in Bacillus thuringiensis
Article Abstract:
CryIIIA gene expression in Bacillus thuringiensis is independent of the key sporulation-specific sigma factors. This was gleaned from a genetic analysis of cryIIIA expression in Bt. It was also found that a different mechanism than that of most of the other cry genes regulates the cryIIIA gene. Unlike the other classes of cry genes, its expression starts during late-exponential growth, and not during sporulation. CryIIIA expression is also increased in a spoOA genetic framework.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Characterization of plasmid pAW63, a second self-transmissible plasmid in Bacillus thuringiensis subsp. kurstaki HD73
Article Abstract:
The plasmid pAW63, the minor plasmid of the two large plasmids of Bacillus thuringiensis subspecies kurstaki strain HD73 was shown to be self-transmissible. Further, it can be transmitted to the other species of the genus Bacillus. The plasmid was tagged with the tetracycline resistance gene and was observed to be transmitted to all recipients within two hours. Bacillus thuringiensis subspecies kurstaki strain HD73 colonizes the larvae of lepidopterans.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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- Abstracts: Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption
- Abstracts: Determination of a 12 kb nucleotide sequence around the 76 degree region of the Bacillus subtilis chromosome. Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees region of Bacillu subtilis chromosome containing genes for trehalose metabolism and acetoin utilization
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- Abstracts: Cross-resistance of the diamondback moth indicates altered interactions with domain II of Bacillus thuringiensis toxins. part 2
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