Development of Bacteroids 16S rRNA gene TaqMan-based real-time PCR assays for estimation of total, human, and bovine fecal pollution in water
Article Abstract:
A real-time PCR assay was designed to target Bacteroides species (AllBac) present in human, cattle and equine feces and direct PCR amplification (without DNA extraction) using the AllBac aasay was tested in feces diluted in water. The results suggested that real-time PCR assays without DNA extraction can be used to quantify fecal concentrations and provide preliminary fecal source identification in watersheds.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Development of goose- and duck-specific DNA markers to determine sources of Escherichia coli in waterways
Article Abstract:
Pool genomic tester and driver DNAs in suppression subtractive hybridizations to enrich for host source-specific DNA markers for Escherichia coli originating from locally isolated geese is studied and used and seven markers were identified. The combined probes identified 73% of the duck isolates examined, indicating they might be useful for determining the contribution of waterfowl to fecal contamination.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Genetic diversity of Escherichia coli isolated from urban rivers and beach water
Article Abstract:
The genetic profiles of Escherichia coli strains found in stormwater, isolated from urban rivers and beach waters where fecal pollution is derived from multiple uncharacterized host sources, were evaluated. The results indicate that understanding the ecology of indicator bacteria is very important while interpreting fecal pollution assessments and developing source detection methodology.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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