Development of a lactococcal integration vector by using IS981 and a temperature-sensitive lactococcal replication region
Article Abstract:
Gene stability in milk fermentation prevents plasmid loss and consequently, lactococcus starter failure. Vectors are constructed from plasmids.However, they do not replicate in lactococci. Developing a lactococcalintegration vector is done by constructing it in such a way that it would be able to integrate genes into the lactococcal chromosome by Campbell-like integration. pKMP10, the lactococcal integration vector constructed in this study, consists of three components: the replication and temperature-sensitive maintenance regions from pSK11 which is a lactose plasmid; an internal fragmentof IS981 which is a lactococcal insertion sequence as the region for homologousrecombination; and the erythromycin resistance and Escherichia coli replicationregion from pVA891, an encoding erthromycin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Characterization of the highly autolytic Lactococcus lactis subsp. cremoris strains CO and 2250
Article Abstract:
The autolytic properties of the Lactococcus lactis subsp cremoris strains 2250 and CO were characterized. Maximum lysis were observed when the strains were grown in M17 broth with 0.4%-0.5% glucose concentration. Autolysis was avoided when the strains were grown in M17 with 1% glucose and rapid lysis of CO occurred when sodium fluoride was introduced in the broth. Results indicate the existence of at least two autolytic enzymes in the strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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