Development of a self-cloning system for Actinomadura verrucosospora and identification of polyketide synthase genes essential for production of the angucyclic antibiotic pradimicin
Article Abstract:
A novel system, based on the regeneration and reliable protoplasting conditions of Actinomadura verrucosospora, was designed in an effort to determine the gene type which codes for the synthesis of angucyclic antibiotic pradimicin. The system generated encouraging results when applied to actual sequence analysis functions, recording an efficiency rate of more than 10(super 5) CFU/microgram of DNA. A close correlation between the nucleotide sequence of PKS genes with angucyclic antibiotic biosynthesis was also observed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the actinomycete Streptomyces coelicolor A3(2)
Article Abstract:
The adenosine 5'-triphosphate (ATP)-phosphofructokinase (PFK) protein was purified to homogeneity from Streptomyces coelicolor A3(2) strain MT 1109 grown under standard conditions by gel filtration technique. The pure, active 110 kDa enzyme exhibited Michaelis-Menten kinetics with a single 40 kDa subunit. The microbial ATP-FTK protein which is allosterically inhibited by phosphoenolpyruvate also exhibited an amino acid sequence that is 70% similar to the PPi-FTK of Amycolatopsis methanolica.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Biphenomycin A production by a mixed culture
Article Abstract:
The taxonomy of the partner strain ofthe mixed culture in the simulated production of biphenomycin A and the role ofeach microorganism in the mixed culture are discussed. High-performance liquid chromatography was used for the taxonomic study of the strain which was isolated from soil samples. Only a small amount of biphenomycin A was produced by Streptomyces griseorubiginosus.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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- Abstracts: Development of a gene transfer system for curing of plasmids in the marine fish pathogen Vibrio salmonicida. Development and use of field application vectors to express nonadaptive foreign genes in competitive environments
- Abstracts: A genetic evaluation of mating system and determinants of individual reproductive success in atlantic salmon. The evolution of bourgeois, parasitic, and cooperative reproductive behaviors in fishes
- Abstracts: Development of monoclonal antibodies that identify Vibrio species commonly isolated from infections of humans, fish, and shellfish
- Abstracts: Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental Antarctica
- Abstracts: Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins