Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental Antarctica

Article Abstract:

Titrated thymidine incorporation (TTI) into DNA is an effective method for the determination of bacterial production in Antarctica soils with favorable moisture conditions. Bacterial productivity in nunatak soils at 10 degrees celsius is less than that in less extreme environmental conditions. There are patches of high productivity and soils near bird nests have higher bacterial activity than those under mosses. The C14 leucine estimation results are similar to the TTI results when the samples are incubated for 3 hrs. Incubation for more than 3 hours gives inaccurate results.

Usage, Measurement, Natural history, Soil microbiology, Antarctic regions, Biological productivity, Thymidine

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Changes in the size and composition of intracellular pools of nonesterified coenzyme A and coenzyme A thioesters in aerobic and facultatively anaerobic bacteria

Article Abstract:

The size and composition of the intracellular pools of nonesterified coenzyme A (CoASH) and coenzyme A (CoA) thioesters in aerobic and facultatively anaerobic bacteria differ significantly. The pools of CoA in aerobic bacteria change hardly and are significantly smaller, while the facultatively anaerobic bacteria CoA pools change quickly in response to the carbon and energy source. The ratio of acetyl-CoA/CoASH in facultatively anaerobic bacteria are significantly higher than those in aerobic bacteria.

Analysis, Bacteria, Aerobic, Aerobic bacteria, Anaerobic bacteria, Esters, Coenzymes

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