Development of a universal microarray based on the ligation detection reaction and 16S rRNA gene polymorphism to target diversity of cyanobacteria
Article Abstract:
The development and validation of a microarray for the identification of cyanobacteria in aquatic environments is described with the help of a ligation detection reaction coupled to a universal array. The findings indicate that a high discriminative power and sensitivity to 1 fmol of the PCR-amplified 16S rRNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Diversity of toxic and nontoxic Nodularia isolates (Cyanobacteria) and filaments from the Baltic Sea
Article Abstract:
Researchers show that Nodularia spumigena, N. baltica, and N. litorea are really one species based on an analysis of the 16S-23S rRNA internally transcribed spacer and the phycocyanin operon intergenic spacer. These organisms are planktic and produce a pentapeptide hepatotoxin called nodularin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Quantitative real-time PCR detection of toxic Nodularia cyanobacteria in the Baltic Sea
Article Abstract:
A specific and sensitive quantitative real-time PCR (qPCR) method is developed for targeting the hepatotoxin nodularin synthetase gene subunit F (ndaF). This method is used to detect the ndaF containing genomes of nodularin-producing Nodularia cyanobacteria of the Baltic Sea.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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