Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes

Article Abstract:

Research was conducted to examine the taxonomic, physiological and genetic diversity of bacteria with the ability to degrade polyaromatic hydrocarbons that were isolated from wastewater and soil samples. Majority of isolates were affiliated to the genera Sphingomonas and Pseudomonas but it had to be taken into account that the selected isolation strategy may have favored Pseudomonas and Sphingomonas strains. Results indicate that Pseudomonas and Sphingomonas strains showed considerably higher activities than the other isolates.

Research, Analysis, Usage, Biodegradation, Bacteria, DNA probes, Microbial enzymes, Hydrocarbons, Aromatic compounds

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Physiological function and regulation of flavocytochrome c3, the soluble fumarate reductase from Shewanella putrefaciens NCIMB400

Article Abstract:

Shewanella putrefaciens is able to utilize alternative terminal electron acceptors during anaerobic metabolism. One of these electron acceptor is fumarate. The facultative anaerobic bacteria is able to utilize fumarate as a terminal electron acceptor because of the presence of novel flavocytochromes the most ubiquitous of which is flavocytochrome c3, a fumarate reductase encoded in the fccA gene. Deletion fccA mutants are unable to utilize fumarate as a terminal electron acceptor.

Physiological aspects, Gram-negative bacteria, Cytochrome c, Fumaric acid

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