Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase

Article Abstract:

Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase. This is done by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins.

Manufacturing processes, Industrial organic chemicals, not elsewhere classified, Wet corn milling, Ethanol, Ethyl Alcohol Manufacturing, Corn Starch, Usage, Production processes, Alcohol, Cornstarch, Alcohol, Denatured

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Construction of a xylan-fermenting yeast strain through codisplay of xylanolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

Article Abstract:

The construction of a yeast strain that hydrolyzes birchwood xylan to xylose is undertaken by codisplaying Trichoderma reesei xylanase II (XYNII) and Aspergillus oryzae beta-xylosidase (XylA) on the cell surface of Saccharomyces cerevisiae. The results show that the direct conversion of xylan to ethanol is accomplished by the xylan-utilizing S. cerevisiae strain.

Genetic aspects, Yeast fungi, Yeasts (Fungi), Microbiological research

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Synergistic saccharification and direct fermentation to ethanol, of amorphous cellulose by use of engineered yeast strain codisplaying three types of cellulolytic enzyme

Article Abstract:

Sustainable energy resource in the world and ethanol produced from biomass is the most widely used biofuel when blended with gasoline. A whole cell biocatalyst, which has the ability to induce synergistic as well as sequential cellulose-degradation reaction was constructed, through codisplay of three types of cellulolytic enzymes.

Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Carbohydrates, Cellulose, Biomass energy

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