High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification
Article Abstract:
A study has been conducted to describe the development of an expression vector for leptin and optimization in induction as related to cell optical density during high-cell density cultivation of recombinant Escherichia coli for the production of gram quantities of recombinant human leptin. Leptin has been identified as a protein secreted by fat cells that influences body weight homeostasis. This study aims to exploit leptin as a regulator of a therapeutic agent to control human obesity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profiling
Article Abstract:
Research discusses genetic approaches toward production enhancement of useful proteins in bacteia as exemplified by the overproduction of human leptin in Escherichia coli. The methodology involves proteome profiling of recombinant E. coli during the overproduction of leptin. The productivity of leptin is achieved by manipulating the cysK gene that has the potential to enhance the production of serine-rich proteins as well.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling
Article Abstract:
A 'second-generation' production strain was derived from a Corynebacterium glutamicum pantothenate producer by rational design to assess its potential to synthesize and accumulate the vitamin pantothenate by batch cultivation. The strategy successfully improved pantothenate production by genetically modified Corynebacterium glutamicum strains and revealed new constraints in attaining high productivity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Transduction, restriction and recombination patterns in Escherichia coli. Sequence comparisons
- Abstracts: Expression of active human tissue-type plasminogen activator in Escherichia coli. RraA: a protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli
- Abstracts: Overproduction of wild-type and bioengineered derivatives of the lantibiotic lacticin 3147
- Abstracts: Heat adaptation alters Escherichia coli O157:H7 membrane lipid composition and verotoxin production. A newly discovered verotoxin variant VT2g produced by bovine verocytotoxigenic Escherichia coli
- Abstracts: Statistical approaches for estimating actinobacterial diversity in marine sediments. Nitrile hydratase and amidase from Rhodococcus rhodochrous hydrolyze acrylic fibers and granular polyacrylonitriles