Discovery of a ptsHI operon, which includes a third gene (ptsT), in the thermophile Bacillus stearothermophilus
Article Abstract:
Bacillus stearothermophilus contains a ptsHI operon with the ptsH, ptsI and ptsT genes required for cellobiose uptake by the phosphoenolpyruvate dependent phosphotransferase system. ptsH encodes a protein similar to the EI of Bacillus subtilis but it has less serine and threonine but more alanine and leucine making the protein more temperature tolerant. The pstI gene encodes a peptide similar to the HPrs of gram-positive bacteria and the ptsT encodes 155 amino acids. The ptsT gene inhibits the growth of ptsI mutants of Escherichia coli at 37 degrees celsius.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Identification and sequencing of the groE operon and flanking genes of Lawsonia intracellularis: use in phylogeny
Article Abstract:
A partial gene library of Lawsonia intracellularis, the bacteria involved in proliferative enteropathy in pigs, was created to examine the bacteria's gene sequence. The gene library was constructed using purified bacteria from pigs to obtain enough DNA. Two seroactive clones, pISI-1 and pISI-2, were obtained after the library was screened with rabbit antisera and raised against L. intracellularis. Sequence analysis showed that operon encoding homologues were of the Escherichia coli chaperonins were present in the pISI-2.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Stringent and growth-rate-dependent control of the gua operon of Escherichia coli K-12
Article Abstract:
The promoter of the gua operon of Escherichia coli K-12 has been located by transcript mapping utilizing primer extension with reverse transcriptase. The surrounding nucleotide sequence has characteristics which are similar to that of promoters under stringent and growth-rate-dependent control. Growth-rate regulation was not affected by guanine-mediated repression. The gua promoter is located back-to-back with the promoter for xseA.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Influence of structural properties and kinetic constraints on Bacillus cereus growth
- Abstracts: Diversity of microorganisms within rock varnish in the Whipple Mountains, California. Microbial community diversity associated with carbon and nitrogen cycling in permeable shelf sediments
- Abstracts: Linkage map of Escherichia coli K-12, edition 10: the physical map. Production of poly(3-hydroxybutyrate) be fed-batch culture of filamentation-suppressed recombinant Escherichia coli
- Abstracts: PCR-ribotyping of Xenorhabdus and Photorhabdus isolates from the Caribbean region in relation to the taxonomy and geographic distribution of their nematode hosts
- Abstracts: Mechanisms of Drosophila retinal morphogenesis: the virtues of being progressive. Cloning and functional analysis of TipE, a novel membrane protein that enhances Drosophila para sodium channel function