Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte
Article Abstract:
Xenopus U8 is a nucleolar snRNA which is implicated in a nucleolytic rRNA processing step other than 18S maturation, and is essential for the maturation of the 5.8S and 2.8S rRNAs at both their 5' and 3' ends. Antisense deoxyoligonucleotides when microinjected into Xenopus oocytes deplete the endogenous pool of U8 RNA, and results in the inhibition of the processing events. These events are responsible for producing mature 28S and 5.8S rRNA at their 5' and 3' ends by the U8-depleted oocytes. The depletion causes accumulation of Novel Nuclear Precursors to 28S and 5.8S rRNA.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Sno storm in the nucleolus: new roles for myriad small RNPs
Article Abstract:
Ribonucleoprotein (RNP) particles in the nucleolus of cells contain snoRNAs that mediate the modification of ribosomal RNA uridines into pseudouridines and the tagging of numerous ribosome moieties with methyl groups. The first snoRNA was identified in 1995 and was named as U3. The ribonucleoprotein was also characterized as the third uridine-rich small RNA of the nucleus. Furthermore, snoRNAs were encoded by introns instead of specific clusters of genes.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro
Article Abstract:
A gene sequence coding for the production of proteins that act in splicing mRNAs that have been generated from DNA was discovered. The spliceosome removes unorthodox sequences from the mrNa through a mechanism that involves direct association of small nuclear ribonucleoproteins (snRNPs) with the sequence to be excised. These snRNPs were observed in vitro to form functional complexes which can be inhibited by the 2'-O-methyl oligonucletoides.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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