Disruption of a silencer domain by a retrotransposon
Article Abstract:
The HML and HMR locus contain silent copies of genes which regulate the mating type of Saccharomyces cerevisiae. The silencing mechanism involves trans-acting factors which recognize sites flanking the repressed genes. Using retrotransposon Ty mutagenesis, mutations that result in the expression of HML-alpha have been isolated. Analysis of the mutations revealed that they fall in two classes. One class affects the trans-acting regulators, while a novel class of mutations activate HML-alpha by inserting at that locus. Several of these mutations insert multimers of Ty, which may act to physically separate the activated gene from the regulatory site.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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DNA synthesis errors associated with double-strand-break repair
Article Abstract:
Repair of a site-specific double-strand DNA break increases the frequency of reversion of an allele next to the site of cleavage, and recombinational repair is mutagenic. Synthesis of DNA is necessary for the processes of DNA damage, duplication and genetic recombination. A number of DNA polymerases are used by cells to perform a number of tasks. DNA synthesis involves a duplication process followed by a correction mechanism to edit insertion errors. It is error free if it involves removal of a lesion and copying an undamaged part and error bound if it involves synthesis across the lesion.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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Ty element-induced temperature-sensitive mutations of Saccharomyces cerevisiae
Article Abstract:
The retrotransposon marked with HIS3, TyH3HIS3 was inserted to to the genome of Saccharomyces cerevisiae to produce temperature-sensitive mutants. The insertions were used to locate genes necessary for growth at high temperatures but are quiescent at low temperature. Five temperature-sensitive mutations were identified namely, hit1-1, hit2-1, hit2-2, hit3-1 and hit4-1. The hit1-1 mutant had the insertion at chromosome X and caused an aberation in the gene coding for a heat-shock protein.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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- Abstracts: Suppression of spontaneous chromosomal rearrangements by S phase checkpoint functions in Saccharomyces cerevisiae
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