Distinct bacterial communities exist beneath a high Arctic polythermal glacier

Article Abstract:

Terminal restriction fragment length polymorphism (T-RFLP) analysis of the bacterial 16S rRNA genes was carried out to determine the relatedness between the supra-, sub-, and proglacial bacterial communities living in the basal ice, sediment and meltwater in the supra-, sub-, and proglacial environments of John Evans Glacier, Nunavut in Canada. It was found that the subglacial water, basal ice and sediment communities were distinct from those found in the supraglacial meltwater and proglacial sediments and the subglacial community seems to have originated from close by environments and adapted to subglacial conditions.

Canada, Science & research, Research, Environmental aspects, Marine bacteria, Water chemistry, Glaciers, Marine microbiology

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Identification of bacterial cells by chromosomal painting

Article Abstract:

A bacterial chromosomal painting (BCP) process was developed for the in situ identification of bacteria by utilizing chromosomal paints constructed from bacterial genomic deoxyribonucleic acid (DNA). The method involves the hybridization of the chromosomal paints to aldehyde-fixed, permeabilized bacterial cells to determine the metabolic and genetic potentials of uncultured and numerically important bacteria. Furthermore, the BCP technique was efficiently utilized for the reciprocal comparisons of distantly related members of Proteobacteria.

Bacteria, Identification and classification, Chromosomes, Microbial genetics

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Comparison of microbial community compositions of two subglacial environments reveals a possible role for microbes in chemical weathering processes

Article Abstract:

The microbial community compositions of samples from two glaciers overlying differing bedrock are compared. Bulk meltwater chemistry indicate that sulfide oxidation and carbonate dissolution account for 90% of the solute from Bench Glacier, Alaska, whereas gypsum/anhydrite and carbonate dissolution accounts for the majority of the flux from John Evans Glacier, Ellesmere Island, Nunavut, Canada.

Cladistic analysis, Oxidation-reduction reaction, Oxidation-reduction reactions, Assaying

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