Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA

Article Abstract:

Amplified ribosomal DNA restriction analysis can be used in the approximation of phylogenetic diversity as well as the genetic composition of microbial communities in different habitats. The procedure identifies both the number and types of dominant ribotypes in the community. This method can also be used to determine community genetic structure and compare different communities on this basis. As such, it provides a simple method to determine biodiversity and changes in composition in microbial communities.

Usage, Genetic aspects, Polymerase chain reaction, Biological diversity, Biodiversity

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Plasmid donor affects host range of promiscuous IncP-1beta plasmid pB10 in an activated-sludge microbial community

Article Abstract:

A study was conducted to show that the host range of a broad-host-range plasmid within an activated-sludge microbial community was influenced by the donor strain and that various mating conditions and isolation strategies increased the diversity of transconjugants detected. Results indicate that the spectrum of hosts to which a promiscuous plasmid transfers in a microbial community can be strongly influenced by the donor from which it transfers

Science & research, Genetic research

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Characterization of diverse 2,4-dichlorophenoxyacetic acid-degradative plasmids isolated from soil by complementation

Article Abstract:

Seven types of 2,4-dichlorophenoxyacetic acid (2,4-D) plasmids were obtained from soil treated with 2,4-D. The catabolic degradation of 2,4-D was encoded as genes by these plasmids which can be transferred to other bacteria. In the host bacteria the 2,4-D degradation is the carbon and energy source. The number of plasmid strains in soils that have been exposed to 2,4-D for a longer time is more than that seen in controlled soil.

Microbial metabolism

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