Evaluation of in vivo activation of protein kinase A under non-dissociable conditions through the overexpression of wild-type and mutant regulatory subunits in Saccharomyces cerevisiae

Article Abstract:

Overexpression of wild-type and mutant regulatory subunits in Saccharomyces cerevisiae has been used for evaluation of in vivo activation of protein kinase A under non-dissociable conditions. This overexpression is a new approach. Biochemical and genetic results support the hypothesis that within the cell it is possible to catalytically active, cAMP-bound, undissociated protein kinase (PKA) holoenzyme.

Argentina, Reports, Physiological aspects, Gene mutations, Gene mutation, Microbial enzymes, Yeast, Saccharomyces, Yeast (Food product), Cellular control mechanisms, Cell regulation, Protein kinases, Microbiological research

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Osmoprotection of Escherichia coli by peptone is mediated by the uptake and accumulation of free proline but not of proline-containing peptides

Article Abstract:

In Escherichia coli osmoprotection by peptone is affected by uptake/accumulation of free proline, but proline-containing peptides do not act in the same way. Meat peptone type I and its effect of E. coli cell growth under hyperosmotic stress has been studied. Peptone enhances growth of E. coli cells in high-osmolarity medium to levels higher than those achieved with the main compatible solute in bacteria, glycine betaine. Natural components in food undermine efforts for food preservation even when water activity is low in some cases. Mechanisms differ from organism to organism.

United Kingdom, Scotland, Environmental aspects, Food, Protection and preservation, Escherichia coli, Food preservation, Fluid-electrolyte balance, Osmoregulation

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