Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains
Article Abstract:
An evaluation of the resolution of the polymerase chain reaction (PCR)-mediated typing techniques indicates that no single technique is sufficient for the discrimination of Saccharomyces cerevisiae strains. The random amplified polymorphic assay using the OPA-11 and 24 primers and PCR fingerprinting allow for the study of genetic diversity between the strains. Restriction enzyme analysis of PCR-amplified units is useful in determining the intraspecies-specific patterns.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores
Article Abstract:
Nested polymerase chain reaction (PCR) is better for detecting fungal spores in air samples than single-step PCR or PCR followed by Southern blotting. Disrupting the spores before PCR analysis was also more effective in maximizing counts than using untreated spores.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities
Article Abstract:
A method is described for estimating the number of viable fungal cells in small samples of liquid culture. It uses a colorimetric method and is faster and more accurate than hemocytometer counting.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Variation of electrophoretic karyotypes in genetically different strains of Saccharomycopsis fibuligera and Yarrowia lipolytica
- Abstracts: Construction of a sorbitol-based vector for expression of heterologous proteins in Saccharomyces cerevisiae. Partial deletion of the Saccharomyces cerevisiae GDH3 gene results in novel starvation phenotypes
- Abstracts: Overproduction of threonine by Saccharomyces cerevisiae mutants resistant to hydroxynorvaline. Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor hap4p
- Abstracts: Impact of carbon and flooding on the metabolic diversity of microbial communities in soils. Metabolic activity of permafrost bacteria below the freezing point
- Abstracts: Evaluation of colicins for inhibitory activity against diarrheagenic Escherichia coli strains, including serotype 0157:H7