Evaluation of the ability of primary selective enrichment to resuscitate heat-injured and freeze-injured Listeria monocytogenes cells
Article Abstract:
The Listeria enrichment broth (LEB) is the primary selective enrichment medium used to detect Listeria monocytogenes contamination in food and environmental samples. The resuscitation rates of injured Listeria cells in LEB and Trypticase soy broth (TSB) containing 0.6% yeast extract were compared. The results showed that growth of heat- and freeze-injured Listeria cells in TSB was superior, leading to cell recovery within 6 to 8 hours. Therefore, the enrichment procedure should be performed only after samples are incubated in nonselective conditions, to allow the recovery of injured cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Evaluation of a new culture media for rapid detection and isolation of Salmonellae in foods
Article Abstract:
Food samples can be tested using Salmosyst broth and Rambach agar (RA) for the presence of Salmonella enteritidis in 48 hours while the conventional method takes at least 4 days. For artificially contaminated samples the sensitivity of both the methods is the same, but the Salmosyst-RA method is more sensitive to Salmonella in naturally contaminated food. The red colonies of Salmonella are easily detected because of the use of RA and the serovars which cannot be detected by the Salmosyst-RA method are not usually pathogenic.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR
Article Abstract:
Reverse transcription-polymerase chain reaction was employed to detect viacle cells of Listeria monocytogenes in meat products. Three genes were assessed to determine their suitability as targets for amplification. Based on this study, the gene iap was selected for amplification in the reverse transcription-polymerase chain reaction because of its specificity for Listeria and its high yield. The test was validated using artificially contaminated meat products.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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- Abstracts: Development of a repair-enrichment broth for resuscitation of heat-injured Listeria monocytogenes and Listeria innocua
- Abstracts: Effects of several factors of the heat-shock-induced thermotolerance of Listeria monocytogenes. Cold shock and its effect on ribosomes and thermal tolerance in Listeria monocytogenes
- Abstracts: Hemolytic activity reevaluation of putative nonpathogenic Listeria monocytogenes strains. Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene
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