Expression of active human tissue-type plasminogen activator in Escherichia coli
Article Abstract:
A study was conducted to show that engineering the disulfide bond machinery of the cell through the expression of periplasmic cysteine oxidoreductases supported the production of active full-length tissue plasminogen activator (tPA). Results indicated several ways for increasing the expression of active, full-length tPA. Findings also correlated the oxidative folding of eukaryotic proteins in the periplasmic space with the isomerization of mismatched disulfides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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The LDL receptor-related protein LRP6 mediates internalization and Lethality of Anthrax toxin
Article Abstract:
A demonstration that low density lipoprotein receptor related protein 6 (LRP6), known to be a coreceptor for the Wnt signaling pathway, is required for anthrax toxin lethality in mammalian cells is presented. The results reported indicate that the LRP6 protein, which was known principally as a mediator of the Wnt signaling pathway, has a hitherto unsuspected role in the delivery of anthrax toxins into cells.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2006
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RraA: a protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli
Article Abstract:
Research demonstrates that the 17.4 kilodalton regulator of ribonuclease activity A, RraA, binds to ribonuclease E and inhibits the latter's endonucleolytic cleavages. Data indicate that RraA abundance circumvents an autoregulatory mechanism that maintains the cellular concentration of ribonuclease E leading to a genome-wide accumulation of ribonuclease E-targeted transcripts.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2003
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- Abstracts: Transduction, restriction and recombination patterns in Escherichia coli. Sequence comparisons
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