Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing
Article Abstract:
A kit method devised for purification of bacterial artificial chromosomes (BACs) could be adapted for effectively preparing individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria. A protocol is described for using solid-phase reversible immobilization (SPRI) for rapid, efficient and inexpensive isolation of sequencing-quality DNA of individual large, low-copy number plasmids from gram-negative and gram-positive bacterial strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Enantioselective synthesis of S-equol from dihydrodaidzein by a newly isolated anaerobic human intestinal bacterium
Article Abstract:
A chemically synthesized dihydrodaidzein was used as the substrate administered to a newly isolated reshaped, gram-negative, anaerobic bacterium from human feces, which can convert dihydrodaidzein to S-equol. Chiral stationary-phase high-performance liquid chromatography eluted only one metabolite, S-equol, which was produced form a bacterial culture containing a racemic mixture of dihydrodaidzein.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Epoxide formation on the aromatic B ring flavanone by biphenyl dioxygenase of pseudomonas pseudoalcaligenes KF707
Article Abstract:
The first prokaryotic epoxide formation on the aromatic ring structures by biphenyl dioxygenase is presented. It suggests that different substrate configurations may react differently with high Fe oxo species of biphenyl dioxygenase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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