Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses

Article Abstract:

Flow cytometry not only provides an efficient and high speed method for screening heterogeneous microbial populations, but also incorporates the benefits of multiparameter data acquisition, multivariate data analysis, high speed analysis and effective cell sorting. Flow cytometry facilitates the estimation of multiple determinants on each cell so as to distinguish between cell types in mixed populations. This technique also enables assessment of bacterial vitality/viability and physiological analysis of the individual cells in an increasingly available monoclonal antibody environment.

Analysis, Observations, Flow cytometry, Microbial populations, Cell physiology

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Quantitative analysis of the physiological heterogeneity within starved cultures of Micrococcus luteus by flow cytometry and cell sorting

Article Abstract:

Cells of the nonsporulating bacterium Micrococcus luteus that are starved for long periods are heterogeneous and have dead, viable, and dormant cells. The cells affect each other during resuscitation and give wrong estimations of the actual number of resuscitable cells. The degree of staining with the fluorescent lipophilic cation rhodamine 123 (Rh123) under starvation conditions reveals the physiological state of the cells. Staining with Rh123 gives two populations, one exhibiting a high degree of fluorescence and another showing a low degree.

Health aspects, Measurement, Bacteria, Fluorescence, Starvation

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Riboflavin production during growth of Micrococcus luteus on pyridine

Article Abstract:

Growth of Micrococcus luteus on media containing pyridine as carbon, nitrogen and energy source results in the production of yellow fluorescent pigments. An investigation was conducted to determine the identity of these pigments. Chromatographic analyses identified three major fluorophores as riboflavin, lumiflavin and lumichrome. These pigments are unlikely to be products of pyridine polymerization. Furthermore, radiotracer experiments confirmed that riboflavin did not arise directly from pyridine.

Microbial metabolism, Identification and classification, Pyridine, Vitamin B2, Riboflavin

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