Functional display of a heterologous protein on the surface of Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A
Article Abstract:
Research was performed to prove that the cell wall anchor of protein A from Staphylococcus aureus is functional in Lactococcus lactis. Evidence suggests that a heterologous anchor sequence can function in a highly-efficient manner to expose functional units at the surface of food-grade microorganism L. lactis. Further studies have to be undertaken as regards the biotechnological applications of this system in antigen presentation, immobilization of enzymatic groups at the surface and immobilization of production strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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The expression of the Photinus pyralis luciferase gene in Staphylococcus aureus Cowan I allows the development of a live amplifiable tool for immunodetection
Article Abstract:
Staphylococcus aureus Cowan I cells, expressing the Photinus pyralis luciferase (luc) gene, produce light which can be used for detecting antigen-antibody complexes by immobilizing the cells on a substrate containing immunoglobulin G. The cells produce a stable glow of light for almost 30 min in the presence of luciferase. The luc gene is present downstream of the S. aureus protein A promoter and fails to affect bacterial growth or plasmid stability.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Comparative and functional analysis of sortase-dependent proteins in the predicted secretome of Lactobacillus salivarius UCC118
Article Abstract:
A comparative bioinformatic analysis of four publicly available Lactobacillus genomes and the genome of Lactobacillus salivarius subsp. salivarius strain UCC118 is performed to identify secreted proteins and those linked to the cell wall. The role in adhesion of the sortase protein and sortase-dependent proteins are examined by using transcriptional analysis and functional characterization of targeted gene knockout mutants.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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