Suppressing posttranslational gluconoylation of heterologous proteins by metabolic engineering of Escherichia coli
Article Abstract:
The effect of overexpression of the phosphogluconolactonase (PGL) enzyme on the specific growth rate, the biomass yield and protein productivity, as well as application of the approach to three unrelated heterologous proteins that display gluconoylation adducts when they are expressed in Escherichia coli BL21 (DE3), is examined. A novel and general strategy is developed for suppressing this nonenzymatic modification by metabolic pathway engineering.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2008
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Karyotypic variation within clonal lineages of the rice blast fungus, Magnaporthe grisea
Article Abstract:
The pathogenic diversification in the rice blast fungus brought about by karyotype variabilitywas studied. Pulsed-gel field electrophoresis were used for analyzing three major clonal populations of Magnaporthe grisea defined by DNA fingerprinting. It was found that neutral genomic arrangements in clones of the fungus are maintained by the lack of sexuality in wild populations reflected by the karyotypic variability in the rice blast fungus.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Identification of an endo-greek small letter beta-1,4-D-Xylanase from Magnaporthe grisea by gene knockout analysis, purification and heterologous expression
Article Abstract:
A Magnaporthe grisea knockout mutant for XYL-6, which was created showed to be as virulent as the parent strain in infecting the rice host and two endoxylanases from the parent were each purified to homogeneity. XYL-6 was introduced into Pichia pastoris for heterologous expression, which resulted in the purification of a fusion protein XYL-6H, establishing that the XYL-6 gene encodes a secreted endo-greek small letter beta-1-4-D-xylanase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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